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Different from some other analysis methods, which depend on a known protein sequence database or a known mass spectrometry database, de novo sequencing uses tandem mass spectrometry for direct analysis based on the.
DE NOVO PROTEIN SEQUENCE ANALYSIS FULL
Here, we presume that the two genes are involved in the formation of primary cell walls and that AmCesA2 is also involved in the formation of secondary cell walls. The de novo antibody protein sequencing platform offers a mass spectrometry solution that accurately obtains the full amino acid sequence without requiring the. De novo sequencing is a method to analyze and identify peptide sequences and some post-translational modified proteins. found by systematic analysis of experimental. The two genes responded to GA₃, 6-BA, and MeJA treatments, and the response to GA₃ was relatively strong. 1027 possible amino acid sequences for compatibility with the design. Real-time RT-PCR showed that both genes were widely expressed in roots, stems, and leaves, but AmCesA2 was more highly expressed in stems. Southern blot analysis showed that multiple copies of AmCesA1 were present in the Acacia mangium genome, but only a single copy of AmCesA2 was present. AmCesA2 had high degrees of similarity with Leucaena leucocephala LlCesA7 and LlCesA8. Cluster analysis showed that AmCesA1 had high degrees of similarity with Glycine max GmCesA1 and Arachis duranensis AdCesA1. AmCesA1 was determined to have six transmembrane regions, and AmCesA2 was determined to have eight transmembrane regions. In silico analysis revealed that AmCesA1 cDNA was 3793 bp in size, had a 3249 bp ORF, and encoded a 1082 amino acid protein AmCesA2 cDNA was 3743 bp in size, had a 3228 bp ORF, and encoded a 1075 amino acid protein. The basis of our sequence selection in the metal cluster donor region of the RC maquette comes from an earlier de novo protein maquette named Due Ferri 3 (DF3) 39,40,41. In this study, two cellulose synthase genes, AmCesA1 and AmCesA2, were cloned from Acacia mangium using transcriptome de novo sequencing analysis and RACE. 85 ISSN: 1614-2942 Subject: Acacia mangium, Arachis duranensis, Glycine max, Leucaena leucocephala, Southern blotting, amino acids, cell walls, cellulose, cellulose synthase, cluster analysis, complementary DNA, fiber quality, gene expression regulation, genes, gibberellic acid, leaves, open reading frames, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, roots, sequence analysis, stems, transcriptome, wood fibers Abstract: Cellulose synthase (CesA) plays a major regulatory role in the cellulose synthesis pathway in plants and is an important factor in controlling wood fiber quality and yield. Cloning and analysis of cellulose synthase genes (CesA) in Acacia mangium Author: Jian Ren, Yuqing Yin, Dian Chen, Yong Wang Source: Tree genetics & genomes 2018 v.14 no.6 pp. Tandem mass spectrometry (MS/MS)-based de novo peptide sequencing is a powerful method for high-throughput protein analysis.